Verification and characterization of chromosome duplication in haploid maize.

نویسندگان

E G de Oliveira Couto

E V Resende Von Pinho

R G Von Pinho

A D Veiga

M R de Carvalho

F de Oliveira Bustamante

M S Nascimento

چکیده

Doubled haploid technology has been used by various private companies. However, information regarding chromosome duplication methodologies, particularly those concerning techniques used to identify duplication in cells, is limited. Thus, we analyzed and characterized artificially doubled haploids using microsatellites molecular markers, pollen viability, and flow cytometry techniques. Evaluated material was obtained using two different chromosome duplication protocols in maize seeds considered haploids, resulting from the cross between the haploid inducer line KEMS and 4 hybrids (GNS 3225, GNS 3032, GNS 3264, and DKB 393). Fourteen days after duplication, plant samples were collected and assessed by flow cytometry. Further, the plants were transplanted to a field, and samples were collected for DNA analyses using microsatellite markers. The tassels were collected during anthesis for pollen viability analyses. Haploid, diploid, and mixoploid individuals were detected using flow cytometry, demonstrating that this technique was efficient for identifying doubled haploids. The microsatellites markers were also efficient for confirming the ploidies preselected by flow cytometry and for identifying homozygous individuals. Pollen viability showed a significant difference between the evaluated ploidies when the Alexander and propionic-carmin stains were used. The viability rates between the plodies analyzed show potential for fertilization.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

-

One of the ways of haploid production is a cross between wheat and maize. Recently success has been achieved in this method, leading to the production of zygotes; these zygotes generally undergo elimination of maize chromosomes and thus lead to production of wheat haploid plant. The purpose of this study was to develop an efficient method of haploid production via maize pollination in short dur...

متن کامل

Development of yellow rust resistant doubled haploid lines of wheat through wheat ´ maize Crosses

In order to produce doubled haploid lines of bread wheat resistant to strip or yellow rust, three F1 wheat hybrids were crossed using pollens of three maize hybrids. Out of 1071 pollinated florets, success in seed set ranged from 63.1% to 93.3% (mean 78%). Differences in seed set among the crosses were not significant. Embryo formation in the seeds developed on different crosses also varied fro...

متن کامل

مقایسه روش‌های تلاقی گندم * ذرت و کشت بساک در تولید گیاهان هاپلویید گندم

The significance of haploid plants as genetic and plant breeding tools has been recognized for a long time. Haploid production techniques including anther culture, isolated microspore culture and intergeneric hybridization between wheat × Hordeum bulbosum and wheat × maize have been used to produce homozygous lines which accelerate breeding programs. In this study, wheat × maize hybridization a...

متن کامل

A Rare Case of Duplication of Chromosome 2 (q31.3q36.3) in a 4.5-year-old Boy and Review of the Literature

De novo duplication of 2q is very rare. Most cases of 2q duplications result from familial translocations, and are associated with simultaneous monosomy of another chromosome segment. To our knowledge and search in English literature there are less than 20 reported cases of isolated 2q duplication. Hereby we introduce a 4.5-year-old Iranian boy of a non-consanguineous marriage who was referred ...

متن کامل